Polyaniline/carbon nanotubes platform for sexually transmitted disease detection |
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Authors: | Renu Singh Chetna Dhand Gajjala Sumana Rachna Verma Seema Sood Rajinder Kumar Gupta Bansi Dhar Malhotra |
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Affiliation: | 1. Department of Science & Technology Centre on Biomolecular Electronics, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi‐110012, India;2. School of Biotechnology, Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi 110006, India;3. Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2‐4, Hibikino, Wakamatsu‐ku, Kitakyushu, 808‐0196, Japan;4. Department of Microbiology, All India Institute of Medical Sciences, New Delhi‐110002, India;5. Centre for NanoBioengineering & Spintronics, Chungnam National University, Daejeon, 305‐764, Korea |
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Abstract: | Polyaniline/carbon nanotubes composite (PANI‐CNT) electrochemically deposited onto indium‐tin‐oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5′‐amino‐labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross‐linker. PANI‐CNT/ITO and aDNA‐Glu‐PANI‐CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT‐IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 × 10?6 M to 1 × 10?17 M with detection limit of 1.2 × 10?17 M. Further, this bioelectrode (aDNA‐Glu‐PANI‐CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non‐Neisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB). Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | polyaniline carbon nanotubes sexually transmitted disease Neisseria gonorrhoeae DNA biosensor |
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