Induction of primordial germ cells from mouse induced pluripotent stem cells derived from adult hepatocytes |
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| Authors: | Masanori Imamura Takashi Aoi Ako Tokumasu Nathan Mise Kuniya Abe Shinya Yamanaka Toshiaki Noce |
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| Institution: | 1. Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan;2. Research Center of Animal Life Science, Shiga University of Medical Science, Shiga, Japan;3. Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan;4. Department of Physiology, School of Medicine, Keio University, Tokyo, Japan;5. Mammalian Cellular Dynamics, RIKEN BioResource Center, Tsukuba, Japan;6. Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan;7. Yamanaka iPS Cell Special Project, Japan Science and Technology Agency, Kawaguchi, Japan;8. Gladstone Institute of Cardiovascular Disease, San Francisco, California;9. Molecular Neuroscience Research Center, Shiga University of Medical Science, Shiga, Japan |
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| Abstract: | Pluripotent stem cells can be established by various methods, but they share several cytological properties, including germ cell differentiation in vitro, independently of their origin. Although mouse induced pluripotent stem (iPS) cells can produce functional gametes in vivo, it is still unclear whether or not they have the ability to produce presumptive germ cells in vitro. Here, we show that mouse iPS cells derived from adult hepatocytes were able to differentiate into presumptive germ cells marked by mouse vasa homolog (Mvh) expression in feeder‐free or suspension cultures. Embryoid body (EB) formation from iPS cells also induced the formation of round‐shaped cells resembling immature oocytes. Mvh+ cells formed clumps by co‐aggregation with differentiation‐supporting cells, and increased expression of germ cell markers was detected in these cell aggregates. Differentiation culture of presumptive germ cells from iPS cells could provide a conventional system for facilitating our understanding of the mechanisms underlying direct reprogramming and germline competency. Mol. Reprod. Dev. 77: 802–811, 2010. © 2010 Wiley‐Liss, Inc. |
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