Dynamics of the Dictyostelium discoideum mitochondrial proteome during vegetative growth,starvation and early stages of development |
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Authors: | Malgorzata Czarna Gregory Mathy Allan Mac'Cord Rowan Dobson Wieslawa Jarmuszkiewicz Claudine M Sluse‐Goffart Pierre Leprince Edwin De Pauw Francis E Sluse |
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Institution: | 1. Laboratory of Bioenergetics and Cellular Physiology, University of Liege, Belgium;2. Laboratory of Bioenergetics, Adam Mickiewicz University, Poznan, Poland;3. Laboratory of Mass Spectrometry, University of Liege, Belgium;4. GIGA‐Neuroscience, University of Liege, Belgium |
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Abstract: | In this study, a quantitative comparative proteomics approach has been used to analyze the Dictyostelium discoideum mitochondrial proteome variations during vegetative growth, starvation and the early stages of development. Application of 2‐D DIGE technology allowed the detection of around 2000 protein spots on each 2‐D gel with 180 proteins exhibiting significant changes in their expression level. In total, 96 proteins (51 unique and 45 redundant) were unambiguously identified. We show that the D. discoideum mitochondrial proteome adaptations mainly affect energy metabolism enzymes (the Krebs cycle, anaplerotic pathways, the oxidative phosphorylation system and energy dissipation), proteins involved in developmental and signaling processes as well as in protein biosynthesis and fate. The most striking observations were the opposite regulation of expression of citrate synthase and aconitase and the very large variation in the expression of the alternative oxidase that highlighted the importance of citrate and alternative oxidase in the physiology of the development of D. discoideum. Mitochondrial energy states measured in vivo with MitoTracker Orange CM?Ros showed an increase in mitochondrial membrane polarization during D. discoideum starvation and starvation‐induced development. |
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Keywords: | 2‐D in‐gel electrophoresis Comparative proteomics Dictyostelium discoideum development Microbiology Mitochondria Mitoproteome |
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