Complex interaction between different proteinaceous components within the cell-wall structure ofCandida albicans |
| |
Authors: | José Luis López-Ribot Deborah A Cortlandt David C Straus K John Morrow W LaJean Chaffin |
| |
Institution: | (1) Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 79430 Lubbock, TX, USA;(2) Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 79430 Lubbock, TX, USA;(3) Present address: Olympia Fields Osteopathic Hospital & Medical Center, 20201 South Crawford Ave., 60461 Olympia Fields, IL, USA |
| |
Abstract: | We have previously described a monoclonal antibody, MAb DC3:H10, which recognized an epitope preferentially expressed on the surface ofCandida albicans germ tubes. In the present study we examined the MAb-reactive material further. Immunoblot analysis of the material purified partially by Sephadex G-200 and DEAE-Sephacel chromatography reacted with antibodies to theC. albicans C3d receptor (CR2). In an ELISA, MAb DC3:H10 captured antigen that was recognized by both anti-CR2 and anti-mp58 fibrinogen binding mannoprotein polyclonal antibodies. The MAb DC3:H10 failed to compete with either of these antisera in an ELISA. Indirect immunofluo-rescence (IIF) analysis showed differences in surface distribution for the MAb DC3:H10, the CR2, and the mp 58 epitopes. Dual labeling IIF experiments showed MAb DC3:H10 binding to be unaffected by binding of fibrinogen or anti-mp58 antibody. However, the binding patterns of MAb DC3:H10 were modified in the presence of anti-CR2 antibody, suggesting a complex interaction between these cell wall components. |
| |
Keywords: | Candida albicans Cell wall Complexes Mannoproteins Receptors |
本文献已被 SpringerLink 等数据库收录! |
|