Immobilization of Horseradish Peroxidase on Multi-Wall Carbon Nanotubes and its Electrochemical Properties |
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| Authors: | Yoon-Mee Lee O-Yul Kwon Yeo-Joon Yoon Keungarp Ryu |
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| Institution: | (1) School of Chemical Engineering and Bioengineering, University of Ulsan, 680-749 Ulsan, Korea;(2) Department of Environmental Engineering, Seoul National University of Technology, 139-743 Seoul, Korea;(3) Division of Nanosciences and Department of Chemistry, Ewha Womans University, 120-750 Seoul, Korea |
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| Abstract: | Horseradish peroxidase (HRP) was immobilized on carboxylated multi-wall carbon nanotubes in the presence of a coupling reagent,
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. The immobilized HRP maintained its oxidative activity for guaiacol over a
broad range of pH values (4–9). An electrode of graphite rod, 6 mm diam. was fabricated using the immobilized HRP. Cyclic
voltammetry of the enzyme electrode confirmed electron transfer between the immobilized HRP and the electrode in the presence
of H2O2 but without an added mediator or a reducing substrate. |
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| Keywords: | carbon nanotubes horseradish peroxidase immobilization nanotubes |
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