Determination and quantification of alpha,beta,gamma,delta-unsaturated aldehydes as pentafluorobenzyl-oxime derivates in diatom cultures and natural phytoplankton populations: application in marine field studies |
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Authors: | Wichard Thomas Poulet Serge A Pohnert Georg |
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Institution: | 1. Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745 Jena, Germany;2. Station Biologique de Roscoff, CNRS, FRE 2775 (Mer et Santé), Place Georges Teissier, F-29682 Roscoff, France |
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Abstract: | Reactive alpha,beta,gamma,delta-unsaturated aldehydes and oxo-acids produced by marine diatoms upon cell damage interfere negatively with the reproduction success of their grazers. A simple, sensitive and specific method based on gas-chromatography coupled to mass spectrometry (EI or CI/EC) was developed for the quantification of these deleterious substances in laboratory diatom cultures and in natural phytoplankton populations. For aldehyde quantification, diatom containing samples are damaged in the presence of O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA.HCl) which leads to an in situ derivatisation without inhibition of the biosynthesis of the aldehydes. The oxime derivates of oxo-acids were in addition reacted with N-tert-butyldimethylsilyl-N-methyl-trifluoracetamide (MTBSTFA). |
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Keywords: | PFBHA·HCl PFBO MTBSTFA 12-ODTE PUA polyunsaturated aldehydes CI/EC chemical ionisation electron capture TRR SC PM AF TPA Decadienal Oxo-acids Marine plankton Chemical defence GC/MS |
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