| Rubisco和RCA在青菜叶绿体中的分布及病毒侵染对其细胞定位的影响 |
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| 引用本文： | 洪健 王卫兵 胡东维 蒋德安 付东亚 陈集双. Rubisco和RCA在青菜叶绿体中的分布及病毒侵染对其细胞定位的影响[J]. 实验生物学报, 2005, 38(1): 29-36 |
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| 作者姓名： | 洪健 王卫兵 胡东维 蒋德安 付东亚 陈集双 |
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| 作者单位： | [1]浙江大学生物技术研究所 [2]浙江大学生命科学学院，杭州310029 |
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| 摘 要： | 运用免疫金标电镜术观察了青菜叶细胞中光合作用关键酶Rubisco和Rubisco活化酶(RCA)的细胞化学定位，结果显示Rubisco和RCA免疫金颗粒主要分布于薄壁组织叶绿体的间质中，在基粒片层上很少，表皮的气孔保卫细胞和维管束薄壁细胞叶绿体内也有分布，在细胞质及线粒体等细胞器中无特异性分布。同时比较观察了感染芜菁花叶病毒(TuMV)的青菜叶绿体Rubisco和RCA免疫金标记结果，发现病组织中结构尚完整的叶绿体Rubisco和RCA标记率略有下降，而结构严重破坏的叶绿体中两种酶标记率分别仅为正常叶绿体的58．44％和64．67％，表明病毒侵染可导致Rubisco和RCA含量下降，影响寄主植物的光合作用。
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| 关 键 词： | 菜叶 叶绿体 青菜 病毒侵染 分布 薄壁细胞 气孔保卫细胞 标记率 RCA 间质 |
Distribution of Rubisco and RCA in Brassica chinensis chloroplasts and effect of TuMV-infection on their cellular localization] |
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| Jian Hong,Wei Bing Wang,Dong Wei Hu,De An Jiang,Dong Ya Fu,Ji Shuang Chen. Distribution of Rubisco and RCA in Brassica chinensis chloroplasts and effect of TuMV-infection on their cellular localization][J]. Acta Biologiae Experimentalis Sinica, 2005, 38(1): 29-36 |
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| Authors: | Jian Hong Wei Bing Wang Dong Wei Hu De An Jiang Dong Ya Fu Ji Shuang Chen |
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| Affiliation: | Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China. jhong@zju.edu.cn |
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| Abstract: | The cellular localizations of Rubisco and Rubisco activase (RCA) in Chinese cabbage (Brassica chinensis L. cv. Suzhou) leaves were investigated by immunogold-labeling electron microscopy. The results showed that Rubisco and RCA were mainly located in chloroplasts of mesophyll, guard cell of stomatal apparatus and parenchyma of vascular bundle. A high density of gold particles were localized preferentially to the chloroplast stroma. In contrast, there were no specific binding of gold particles detected in the cytoplasm, vacuole, mitochondria and other organelles. As infected by turnip mosaic virus, the density of gold particles decreased lightly in the abnormal chloroplasts and dropped by much lower (58.44% and 64.67% as that in the health chloroplasts respectively) in swollen chloroplasts. This indicated that virus infection caused the decreases of Rubisco and RCA in host plant, which then affected plant photosynthesis. |
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