Establishment and characterization of two new human ovarian cancer cell lines UWOV1 and UWOV2 and a subline UWOV2 (Sf) growing in serum-free conditions: Growth characteristics,biochemical, and cytogenetic studies |
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| Authors: | Terry Golombick R Dansey W R Bezwoda Jennifer Rosendorff |
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| Institution: | (1) Department of Medicine, Haematology/Oncology, University of Witwatersrand, 7 York Road, Parktown 2193, Johannesburg, South Africa;(2) Department of Human Genetics MRC Human Ecogenetics Research Unit, University of Witwatersrand, 7 York Road, Parktown 2193, Johannesburg, South Africa |
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| Abstract: | Summary The establishment, growth, and characterization of two new continuously growing human ovarian cancer cell lines (UWOV1 and
UWOV2) as, well as a subline (UWOV2, Sf) grown in chemically defined, serum-free medium are described. The cell lines were
derived from ascitic tumors of two patients suffering from cystadenocarcinomas of the ovary. Both UWOV1 and UWOV2 lines grow
in anchorage-dependent fashion as monolayers, whereas UWOV2 (Sf) forms multilayered domelike structures. Cytogenetic studies
revealed nonrandom abnormalities involving chromosomes 1 and 11 in all three cell lines. Secretion of soluble collagen was
detected in all three cell lines. In addition, UWOV2 (Sf) produces and secretes large amounts of extracellular matrix material
with an ordered fibrillar structure which may function as an attachment factor for the serum-free cells. These cell lines
seem to be useful for further studies of the biology of human ovarian cancer.
This research was supported by grants from National Cancer Association (S. A.) and Bekker Trust Foundation. |
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| Keywords: | ovarian cancer cell line serum-free culture |
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