正常人耵聍中马拉色菌菌种构成研究

目的研究正常人耵聍中马拉色菌菌种构成及同一宿主耵聍中菌种是否一致。方法采集45名健康志愿者双侧耵聍,0.1%曲拉通X-100溶解稀释后接种于含菜籽油培养基,生化及形态学方法鉴定到种,同时提取菌种DNA,用真菌通用引物ITS1/ITS4做PCR扩增并测序鉴定。结果有44例(97.78%)双侧耵聍中均培养出马拉色菌(共分离出88株菌),菌种构成:糠粃马拉色菌29株(32.95%)、斯洛菲马拉色菌23株(26.14%)、合轴马拉色菌18株(20.45%)、球形马拉色菌11株(12.50%)、限制性马拉色菌7株(7.95%)。44例(88株菌)中双侧耵聍菌种相同者有38例(76株菌)(一致率86.36%)。结论正常人耵聍中马拉色菌菌种分布较广,主要菌种为糠秕马拉色菌。同一宿主双侧耵聍中马拉色菌菌种具有一定的一致性。

Constitution of malassezia species in normal human cerumen

Objective To investgate the constitution of Malassezia species in normal human cerumen and analyze the concordance of Malassezia species in two sides' external auditory canals from the same host.Methods Forty-five healthy volunteers were included.The cerumen were diluted and dissolved completely with 0.1%Triton-X 100 and seeded onto medium containing rapeseed oil.The isolates were identified to species through physiological and morphological characteristics.They were also identified by amplifying the ITS region of rDNA,followed by DNA sequence analysis of the PCR products.Results Malassezia spp.were isolated from 44 of 45 individuals studied(97.78%)in there two sides' external auditory canals.Five species were identified from 88 isolated strains,including 29 strains(32.9%)of M.furfur,23 strains(26.14%)of M.slooffiae,18 strains(20.45%)of M.sympodialis,11 strains(12.50%)of M.globosa and 7 strains(7.97%)of M.restricta.Among 44 volunteers,isolates of Malassezia from each individual of 38 volunteers shared the same species(86.36%).Conclusions The species of Malassezia is variegated in cerumen of human and the dominant species is M.furfur.In two sides' cerumen from the same host,the species of Malassezia tend to be consistent.