Effect of ionizing radiation on multidrug resistance of human larynx cancer HEp-2 cells |
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| Authors: | V V Shaposhnikova A F Korystova M O Emel’yanov L N Kublik A A Kudryavtsev M Kh Levitman Yu A Kim Yu N Korystov |
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| Institution: | (1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia;(2) Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia |
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| Abstract: | Effects of ionizing radiation on multidrug resistance (MDR) of human larynx cancer HEp-2 cells have been studied. MDR was determined from sensitivity of HEp-2 cells to daunorubicin, taxol and vincristine in the absence and in the presence of MDR inhibitors (cyclosporin A and avermectin B1) and from the suppression by cyclosporin A of the rhodamine-123 release from HEp-2 cells. It was found that 8 and 16 h after irradiation (4 Gy), HEp-2 MDR was increased with a further return to the control level by the 24th hour after irradiation. The effect of irradiation was especially well-pronounced by 16 h for cells irradiated with 1 Gy and was manifested in enhanced release of rhodamine-123 and increased resistance of HEp-2 cells to vincristine. Besides, this effect depended on cell density, being at maximum at 80–100 × 103 cells/cm2. It is concluded that the observed dependence of HEp-2 MDR on ionizing radiation and cell density is a result of changes in intracellular content of reactive oxygen species. |
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