Characterization of Mitotic Cycles Preceding Xylogenesis in Cultured Explants of Helianthus tuberosus L. |
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Authors: | PHILLIPS R |
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Institution: | Department of Botany and Microbiology, University College London Gower Street, London WC1E 6BT |
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Abstract: | The number of mitotic cycles intervening between the transferof dormant Helianthus tuberosus (Jerusalem artichoke) tuberexplants to culture medium and the differentiation of the firsttracheary elements at 48 h was investigated by a pulse-labellingtechnique employing quantitative autoradiography. Silver-graincounts indicated that differentiation was preceded by threemitotic cycles Duration of the cell cycle phases were estimatedby a pulse-labelling method. From calculation of the phase durationsit was estimated that the first visible signs of tracheary elementdifferentiation occurred from 710 h after the last mitosis. Helianthus tuberosus L., Jerusalem artichoke, tracheary elements, cultured explants, tissue culture, mitotic cycles, cell cycle, tritiated thymidine, autoradiography |
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