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Molecular identification of five Indian sciaenids (pisces: perciformes,sciaenidae) using RAPD markers
Authors:Wazir S. Lakra  M. Goswami  V. Mohindra  K. K. Lal  P. Punia
Affiliation:(1) National Bureau of Fish Genetic Resources, Lucknow, 226 002, India
Abstract:The randomly amplified polymorphic DNA (RAPD) markers were used to detect interspecific genetic variability and genetic relatedness among five Indian sciaenids namely Otolithes cuvieri, Johnieops sina, Johnieops macrorhynus, Johnieops vogleri and Protonibea diacanthus for the first time. Eight RAPD primers (OPA01, OPA06, OPA07, OPA18, OPP12, OPP14, OPP16 and OPP11) generated 40 species specific diagnostic bands. The highest genetic divergence was detected between J. macrorhynus and P. diacanthus (0.586) where as the lowest one was observed between J. sina and J. vogleri (0.274). Handling editor: C. Strumbauer
Keywords:Sciaenids  RAPD  Molecular Identification
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