Molecular identification of five Indian sciaenids (pisces: perciformes,sciaenidae) using RAPD markers |
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Authors: | Wazir S. Lakra M. Goswami V. Mohindra K. K. Lal P. Punia |
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Affiliation: | (1) National Bureau of Fish Genetic Resources, Lucknow, 226 002, India |
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Abstract: | The randomly amplified polymorphic DNA (RAPD) markers were used to detect interspecific genetic variability and genetic relatedness among five Indian sciaenids namely Otolithes cuvieri, Johnieops sina, Johnieops macrorhynus, Johnieops vogleri and Protonibea diacanthus for the first time. Eight RAPD primers (OPA01, OPA06, OPA07, OPA18, OPP12, OPP14, OPP16 and OPP11) generated 40 species specific diagnostic bands. The highest genetic divergence was detected between J. macrorhynus and P. diacanthus (0.586) where as the lowest one was observed between J. sina and J. vogleri (0.274). Handling editor: C. Strumbauer |
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Keywords: | Sciaenids RAPD Molecular Identification |
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