Chondrocyte-specific phenotype confers susceptibility of rat chondrocytes to lysis by NK cells |
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Authors: | Agata Bia?oszewska Justyna Niderla-Bielińska Anna Osiecka-Iwan Claudine Kieda |
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Institution: | a Department of Histology and Embryology, Center of Biostructure Research, Medical University of Warsaw, Cha?ubińskiego 5, 02004 Warsaw, Poland b Glycobiology of Cell Recognition, Centre de Biophysique Moleculaire, CNRS UPR 4301, University of Orleans, 45071 Orleans CEDEX 2, France |
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Abstract: | Normal chondrocytes are targets for natural killer (NK) cells. Since the mechanism of this phenomenon remains unknown, the present study was aimed at testing whether it is associated with chondrocyte-specific phenotype defined as ability of cartilage cells to produce sulfated glycosaminoglycans (GAG) and express collagen II and aggrecan mRNA. Lysis of rat epiphyseal chondrocytes by syngeneic spleen mononuclear cells (SMCs) was evaluated by 51Cr-release assay. Loss of chondrocyte phenotype following long-term culture resulted in their decreased susceptibility to lysis. Similar effect was also observed after suppression of chondrocyte phenotype by TNF. On the other hand, stimulation of cartilage-specific matrix component synthesis by IGF-1 resulted in increased chondrocyte killing and exogenous chondroitin sulfate A stimulated NK cell-mediated cytotoxicity against chondrocytes and human K562 cells. This suggests that chondrocyte susceptibility to lysis by NK cells depends on chondrocyte-specific phenotype, especially sulfated GAG production. |
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Keywords: | Chondrocytes Chondroitin sulfate Glycosaminoglycans Natural killer cells Natural cell-mediated cytotoxicity |
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