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Effect of PEG and other additives on cyclodextrin production by Bacillus macerans cyclomaltodextrin-glycosyl-transferase
Authors:M. F. Delbourg  Ph. Drouet  F. De Moraes  D. Thomas  J. N. Barbotin
Affiliation:(1) Laboratoire de Technologie Enzymatique. U.R.A., 1442 du CNRS. U.T.C., BP 649, 60206 Compiègne, France
Abstract:Summary The effect of PEG and other polyols additives on cyclodextrins (CDs) production by Bacillus macerons cyclomaltodextrin-glycosyl-transferase (CGTase) was investigated. Mannitol, glycerol and PEG-200 (20%,v/v) enhanced the enzymatic production yield regardless of substrate concentration. Furthermore, the PEG-200 addition increased the thermostability of the CGTase.
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