Sulfide-controlled continuous culture of sulfate-reducing bacteria |
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| Affiliation: | 1. Carnegie Institution of Washington, Geophysical Laboratory, Washington, DC 20015, USA;2. Woods Hole Oceanographic Institution, Biology Department, Woods Hole, MA 02543, USA;1. Department of Nanobiotechnology, Biology Centre, ISB, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic;2. Department of General and Industrial Microbiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd, 1164 Sofia, Bulgaria;3. Laboratory Microwave Magnetics, Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee Blvd, 1784 Sofia, Bulgaria;4. Global Change Research Institute, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic;5. Department of Applied Chemistry, Faculty of Agriculture, University of South Bohemia, Branisovska 1457, 370 05 Ceske Budejovice, Czech Republic;6. Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic;1. Department of Pharmacy, University of Patras School of Health Sciences, Patras, Greece;2. Department of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, Abu Dhabi, United Arabic Emirates;3. Faculty of Medicine and Health Sciences, Department of Pathology, Bioinformatics Unit, Erasmus University Medical Center, Rotterdam, The Netherlands;4. Department of Pathophysiology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece;5. First Department of Dermatology-Venereology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece;6. General Department, University of Thessaly, Karditsa, Greece;7. The Golden Helix Foundation, London, United Kingdom;1. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan;2. Corporative Nihon Mikuniya, 3-25-10 Mizonokuchi, Takatsu, Kawasaki, Kanagawa 213-0001, Japan;1. UNESCO/UNITWIN Wicop, Department of Physical Chemistry, Faculty of Marine and Environmental Science, University of Cádiz, Polígono Río San Pedro S/n, 11510, Puerto Real, Cádiz, Spain;2. Norwegian University of Science and Technology, Department of Chemistry, 7491, Trondheim, Norway;3. C.I.C.A. Ingenieros Consultores Perú S.A.C., Av. Javier Prado Este, 492, San Isidro, Lima, Peru |
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| Abstract: | In continuous culture set-up for sulfate-reducing bacteria a sulfide electrode (made from silver wire) is used to control the electron donor supply and the medium pump. The sulfied concentration of the medium is kept at a low level by continuosly flushing out H2S and replacing it with CO2. The pH is controlled automatically by regulating the CO2 content of the gas mixture flushed through the medium. With the sulfide-controlled set-up sulfate-reducing bacteria can be grown in chemostat culture under electron donor as well as electron acceptor limitation. Furthermore, by continuously washing out the culture to a preselected residual sulfide concentration, cells can be grown in sulfidostat culture under non-limiting conditions at maximal growth rate. Growth yields of Desulfotmaculum orientis, when growth in this system with hydrogen as electron donor, were considerably higher than previously reported. |
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