Recombination between short direct repeats in a RecA host |
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| Authors: | Ian M Jones Sandy B Primrose and Stanislav D Ehrlich |
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| Institution: | (1) Institut de Recherche en Biologie Moléculaire 2, Place Jussieu, Paris;(2) Searle Research and Development, Lane End Road, High Wycombe, U.K.;(3) Present address: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, U.K. |
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| Abstract: | Summary Spontaneous deletion derivitives of plasmid pHV14, a recombinant of pBR322, have been isolated in the recA strain HB101. About 2.5 kilobases (Kb) of DNA has been lost in each of the four plasmids described including, in two cases the region involved in control of copy number and the initiation of replication.Sequence analysis of each deletion junction has revealed that in 3 out of 4 cases the deletion event occured by recombination between short direct repeats of as little as 7 base pairs (bp). |
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