夏枯草多糖的分离纯化与抗氧化活性研究

对夏枯草多糖(PP2)的分离纯化方法作了探讨,通过正交试验,筛选出多糖提取条件的最佳组合。采用纸层析、琼脂糖凝胶电泳和紫外分光光度法对多糖纯度进行鉴定。通过气相色谱仪对多糖的单糖组成进行分析。通过体外化学模拟,研究夏枯草多糖的抗氧化性。实验表明,夏枯草多糖对O2-.、.OH二种自由基及亚硝酸根离子具有一定的清除能力,对R.自由基的清除较弱,具有防止膜脂质过氧化,减少红细胞溶血和降低脂质过氧化产物丙二醛的生成量的作用。

Isolation, Purification and Antioxidant Activity of the Polysaccharide of Prunella vugaris ( Labiatae )

This report described the isolation and purification of the polysaccharide from Prunella vugaris L.(PP2).The best combination of isolation conditions had been obtained through the orthogonal experiment.Paper Chromatography,agarose gel electrophoresis and ultraviolet spectroscopy had been introduced to determine the purity of the PP2 and fractional deposition and gas chromatography had been applied to analyze the monosaccharide constituents of PP2.By the chemical simulation in vitro,the antioxidant activity of PP2 had been studied.The results of the experiment showed that PP2 had significant scavenging effect on oxide radical,hydroxide radical and univalent radical NO_2 but poor scavenging effect on organic radical.PP2 could prevent the membrane lipid from peroxidation and reduce hemolysis of red cells and production of MDA as well.