Structural difference between polymerized and non-polymerized fragment X, obtained by plasmin digest of fibrinogen |
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Authors: | Hiroko Sato Joel K Swadesh |
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Institution: | a Department of Polymer Chemistry, Kyoto University, Yoshida-honmachi, Kyoto 606-01, Japan b Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19104, USA |
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Abstract: | Fragment X (LMrFX) was obtained as low molecular weight preparations from a late stage 2 plasmin digest of human fibrinogen. The thrombin-treated LMrFX preparations, which resulted in impaired polymerization, were further subfractionated into polymerized and non-polymerized components. The fractions were examined by SDS-PAGE and immunochemical methods. In polymerized fractions, more peptide bands were observed on SDS-PAGE in the reduced state than in non-polymerized fractions. Both fractions contained a similar number of internal cleavages in the A , Bβ and γ chains, which are linked by disulfide bonds. Thus, the partial deficiencies in polymerization sites of the carboxy terminal region of the γ chain and the amino terminal portions of the Bβ chain, as well as internal cleavage, were considered to participate in the impairment of the thrombin-induced polymerization of LMrFX. |
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Keywords: | Fibrinogen fragment X plasmin digest antibodies internal cleavage polymerization sites |
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