柞蚕核型多角体病毒PstI-B、C片段克隆及序列分析

克隆并测序分析了柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)PstI-B和C片段。结果表明:ApNPVPstI-B片段长7406bp,编码7个开放阅读框(open readingframes,orf),包括p87、he65、pnk/pnl、odv-ec43基因及黄杉毒蛾核型多角体病毒(Orgyiapseudotsugatamulticapsid nucleopolyhedrovirus,OpMNPV)orf107、orf108同源区。ApNPVPstⅠ-C长6663bp,编码11个orf,包括pk-1、orf1629、polh、lef-2、ptp-2、ctl-1、ptp-1基因及OpMNPVorf5、orf7、orf8和orf11同源区。在鉴定的18个杆状病毒基因中,he65和orf1629基因分化较大;polh和lef-2基因较保守。ApNPV是已知的第3个编码pnk/pnl基因、第4个同时编码ptp-1和ptp-2两个基因的杆状病毒。

Clone and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus PstI-B and C fragments

Antheraea pernyi nucleopolyhedrovirus(ApNPV)PstI-B and C fragments were cloned and sequenced.ApNPV PstI-B was 7406 bp long,contained seven open reading frames(orfs)/genes,including p87,he65,pnk/pnl,odv-ec43 and Orgyia pseudotsugata multicapsid nucleopolyhedrovirus(OpMNPV)orf107,orf108 homologue on either strands of genomic DNA.ApNPV PstI-C was 6663 bp long,contained eleven orfs/genes,including pk-1,orf1629,polh,lef-2,ptp-2,ctl-1,ptp-1 and OpMNPV orf5,orf7,orf8,orf11 homologue on either strands of genomic DNA.Among the eighteen baculovirus genes identification,he65 and orf1629 were two diverse genes,while polh and lef-2 were two conserved genes.ApNPV was the third baculovirus found to contain pnk/pnl gene,the fourth baculovirus found to contain both ptp-1 and ptp-2 gene.