Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in primary astrocyte cultures |
| |
Authors: | Campisi A Caccamo D Li Volti G Currò M Parisi G Avola R Vanella A Ientile R |
| |
Institution: | Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, V.le A. Doria, 6, 95100 Catania, Italy. |
| |
Abstract: | The aim of this study was to evaluate the involvement of oxidative stress in glutamate-evoked transglutaminase (TGase) upregulation in astrocyte cultures (14 DIV). A 24 h exposure to glutamate caused a dose-dependent depletion of glutathione intracellular content and increased the ROS production in cell cultures. These effects were receptor-mediated, as demonstrated by inhibition with GYKI 52466. The pre-incubation with glutathione ethyl ester or cysteamine recovered oxidative status and was effective in significantly reducing glutamate-increased tissue TGase. These data suggest that tissue TGase upregulation may be part of a biochemical response to oxidative stress induced by a prolonged exposure of astrocyte cultures to glutamate. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|