Trans-splicing of an artificially split fungal mini-intein |
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Authors: | Elleuche Skander Pöggeler Stefanie |
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Institution: | Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August-University of G?ttingen, Germany. |
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Abstract: | Inteins are internal protein domains found inside the coding region of different proteins. They can autocatalytically self-excise from their host protein and ligate the protein flanks, called exteins, with a peptide bond via a post-translational process called protein cis-splicing. In contrast, protein trans-splicing involves inteins split into an N- and a C-terminal domain. Both domains are synthesized as two separate components and each joined to an extein; the intein domains can reassemble and link the joined exteins into one functional protein. In this study, we introduced three split sites into the PRP8 mini-intein of Penicillium chrysogenum and demonstrated for the first time trans-splicing of a fungal PRP8 intein. Two of the sites introduced allowed splicing to occur in trans while the third was not functional. |
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Keywords: | Intein Protein splicing Penicillium chrysogenum PRP8 protein Trans-splicing |
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