Constitutive expression of the neutral PR-5 (OLP, PR-5d) gene in roots and cultured cells of tobacco is mediated by ethylene-responsive cis-element AGCCGCC sequences |
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| Authors: | S Kitajima T Koyama Y Yamada F Sato |
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| Institution: | (1) Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan Fax: +81-75-7536398 e-mail: fumihiko@kais.kyoto-u.ac.jp, JP;(2) Graduate School of Biological Science, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0101, Japan, JP |
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| Abstract: | The constitutive accumulation of tobacco neutral PR-5 (osmotin-like protein; OLP, PR-5d) in roots and cultured cells was
studied in transgenic tobacco plants harboring the OLP promoter::GUS gene. This construct showed strong β-glucuronidase expression in vascular tissues and cortex of roots as well as in cultured cells. Analysis using a mutated promoter
showed that ethylene-responsive elements (AGCCGCC) were necessary for constitutive expression in roots and cultured cells.
An electrophoretic mobility shift assay indicated that ERF3 (EREBP3), an ethylene-responsive-element-binding factor that was
reported to be expressed in roots and in cultured cells as well as in ethephon-treated leaves, could bind to the AGCCGCC sequences
of the OLP gene. These findings suggest that AGCCGCC sequences and ERFs mediate the constitutive expression of the OLP gene
in roots and cultured cells of tobacco.
Received: 14 November 1997 / Revision received: 29 May 1998 / Accepted: 8 July 1998 |
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| Keywords: | Callus ERF (EREBP) Osmotin-like protein Root Tobacco |
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