Replication of mycoplasmavirus MVL51: I. Replicative intermediates. |
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Authors: | J Das J Maniloff |
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Institution: | Departments of Microbiology and of Radiation Biology and Biophysics University of Rochester Rochester, New York 14642, USA |
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Abstract: | The intracellular replication of the single stranded DNA of the non-lytic bullet-shaped Group L1 mycoplasmavirus, MVL51, has been shown to involve three virus specific DNAs: RFI, RFII and SS. The relative sedimentation rates and ethidium bromide CsCl gradient analysis show that RFI is covalently closed circular double stranded DNA and RFII is a nicked form of RFI. SS is circular single stranded progeny viral DNA. RFI and RFII serve as precursors for the synthesis of progeny SS. |
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