Function of the iron-binding chelator produced by Coriolus versicolor in lignin biodegradation |
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Authors: | Lu Wang WenChao Yan JiaChuan Chen Feng Huang PeiJi Gao |
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Institution: | (1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China;(2) Shandong Key Lab of Pulp and Paper Engineering, Shandong Institute of Light Industry, Jinan, 250100, China |
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Abstract: | An ultrafiltered low-molecular-weight preparation of chelating compounds was isolated from a wood-containing culture of the
white-rot basidiomycete Coriolus versicolor. This preparation could chelate Fe3+ and reduce Fe3+ to Fe2+, demonstrating that the substance may serve as a ferric chelator, oxygen-reducing agent, and redox-cycling molecule, which
would include functioning as the electron transport carrier in Fenton reaction. Lignin was treated with the iron-binding chelator
and the changes in structure were investigated by 1H-NMR, 13C-NMR, difference spectrum caused by ionization under alkaline conditions and nitrobenzene oxidation. The results indicated
that the iron-binding chelator could destroy the β-O-4 bonds in etherified lignin units and insert phenolic hydroxyl groups.
The low-molecular-weight chelator secreted by C. versicolor resulted in new phenolic substructures in the lignin polymer, making it susceptible to attack by laccase or manganese peroxidase.
Thus, the synergic action of the iron-binding chelator and the lignocellulolytic enzymes made the substrate more accessible
to degradation.
Supported by the National Natural Science Foundation of China (Grant Nos. 30170027 and 30371136) |
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Keywords: | Coriolus versicolor lignin degradation iron binding chelator |
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