Phosphate accumulation of <Emphasis Type="Italic">Acetobacter xylinum</Emphasis> |
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Authors: | Lubov P Ryazanova Natalia E Suzina Tatiana V Kulakovskaya Igor S Kulaev |
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Institution: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow, Russia |
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Abstract: | The cells of Acetobacter xylinum decreased phosphate concentration in the medium from 5 to 2.5 or 0.3 mM during incubation in the presence of Mg2+ and glucose, or Mg2+ and casamino acids, respectively. The prevalence of orthophosphate or polyphosphate in the biomass of A. xylinum depends on the medium composition. Under phosphate uptake in the presence of glucose, the content of orthophosphate in the
biomass changed little, while that of polyphosphate increased fourfold. At incubation with casamino acids, the content of
orthophosphate increased 15 times, while that of polyphosphate increased only 2.5 times. Some part of orthophosphate in this
case seems to be bound with the cell surface. The polyphosphate chain length in the cells of A. xylinim increases under phosphate uptake. This increase is more noticeable in the presence of glucose. Casamino acids can be replaced
by α-ketoglutaric acid in combination with (NH4)2SO4, or arginine, or glutamine, the catabolism of which results in formation of NH4
+ and α-ketoglutarate. |
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Keywords: | Acetobacter xylinum Bacteria Polyphosphate Phosphate Uptake Localization Magnesium |
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