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An immunological assay for chloramphenicol acetyltransferase
Authors:D K Burns  R M Crowl
Affiliation:1. The Volcani Center, Institute of Animal Sciences, A.R.O., Bet Dagan 50250, Israel;2. Animal Improvement Programs Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705-2350, USA;1. Department of Chemistry ‘Ugo Schiff’, via della Lastruccia 3-13, Sesto Fiorentino, 50019, Firenze, Italy;2. Départment de Pharmacochimie Moléculaire, Université Grenoble Alpes, UMR 5063, Grenoble, France;1. MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China;2. Laboratory of Diagnostics Development, China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong, 266032, China;3. Lianyungang Animal Husbandry and Veterinary Station, Lianyungang, Jiangsu, 222003, China;4. Technology Center of Hefei Customs, Hefei, 230022, China;5. China Pharmaceutical University, Nanjing, 211198, China;1. Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2330 RC Leiden, The Netherlands;2. Leiden University Medical Center, Department of Parasitology, Albinusdreef 2, 2330 RC Leiden, The Netherlands;3. Universitas Indonesia, Faculty of Medicine, Department of Parasitology, Salemba Raya 6, 10430 Jakarta, Indonesia;1. Centro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, CEP 96160-000, Brazil;2. Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, CEP 68740-970, Brazil
Abstract:We report the production and utility of rabbit polyclonal antisera to the bacterial protein chloramphenicol acetyltransferase (CAT). The anti-CAT antibodies specifically react with CAT protein produced in bacterial or avian cells. Results reported here demonstrate that detection of CAT by immuno-dot blot techniques is at least as sensitive as the currently used in vitro enzymatic assay. The availability of antibodies to CAT offers a number of potential advantages to investigators who now use the CAT gene to study factors influencing gene regulation.
Keywords:
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