影响被孢霉产生含γ-亚麻酸油脂的几种因素

对培养条件，如氮源种类、C／N比率、pH、种子种龄与接种量对被孢霉(Mortierellasp.)M14菌株的细胞生长和油脂形成的影响作了研究。结果表明，二级发酵优于一级发酵。M14菌株二级发酵时，种子种龄以49h为适，接种量宜大(30％)。无机氮源有利于不饱和脂肪酸的产生，有机氮源有利于细胞的增殖。低C／N比率有利于菌丝体产量的提高，提高C／N比率则能促进菌体细胞内的油脂合成。pH对菌体生长、油脂产生以及γ-亚麻酸含量都有显著的影响。

Factors Effecting on Production of Lipid Containing r-linolenic Acid by Mortierella sp.

The influence of cultural conditions, such as nitrogen source, C/N ratio, pH, age and volume of inoculum on cell growth and lipid formation of Mortierella sp. M14 strain was studied. It was showed that 2 steps was better than 1 step fermentation. During 2 steps fermentation with M14 strain, 49h seed age was suitable,and larger seed volume was required (30%). Inorganic nitrogen source contributed to produce unsaturated fatty acid and organic nitrogen source contributed the cell growth of M14. Low C/N ratio facilitate the enhancement of biomass, higher C/N ratio was able to promote the synthesis of lipid in mycelia. The cell growth, lipid formation were also influenced by pH markedly.