青蒿提取物抗单纯疱疹病毒活性研究

用细胞病变效应(CPE)法证明了青蒿水提物具有抗单纯疱疹病毒Ⅱ型(HSV—2)活性，通过初步分离纯化得到抗HSV—2活性的有效成分。用MTT法研究了青蒿水提物和有效成分的细胞毒性和抗HSV—2活性，CC50分别为5．29mg／ml和4，94mg／ml，IC50分别为1．45mg／ml和0．128mg／ml，TI分别为3．65和38．6。以0．5mg／ml的无环鸟苷(ACV)作为阳性对照，结果显示有效成分在体外可以明显抑制HSV—2的致细胞病变作用，效果与ACV相当。

A Study of antiviral activity against HSV-2 of the extract from Artemisia annua L

The anti herpes simplex virus type 2 activity of the crude water extract of Artemisia annua L.was proved by cytopathogenic effect(CPE)method.By re extracting with different solvents,the partially separated components maintaining antiviral activity were obtained.The 50% cytotoxity concentration(CC 50 ),50% inhibition concentration(IC 50 )and therapy index(TI)of the crude water extract and the partially separated components were 4 94 mg/ml,0 128 mg/ml,38 6 and 5 29 mg/ml,1 45 mg/ml,3 65,respectively measured by MTT method with 0 5 mg/ml of ACV as the positive control,showing that the partially separated components have remarkable anti HSV 2 activity in vitro.