Growth and development of fern gametophytes in an airlift fermenter |
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| Authors: | E Sheffield G E Douglas D J Cove |
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| Institution: | (1) 3614 Stopford Building, School of Biological Sciences, University of Manchester, Oxford Road, M13 9PT Manchester, UK;(2) Department of Genetics, University of Leeds, LS2 9JT Leeds, UK;(3) Present address: Department of Cryptogamic Botany, Natural History Museum, Cromwell Road, SW7 5BD London, UK |
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| Abstract: | Spores of the fernsPteridium aquilinum andAnemia phyllitidis were grown in an airlift fermenter and subsequent growth and development of gametophytes was monitored. Both species produced greater biomass than that generated in any other solid- or liquid-based culture system tested.Pteridium generated more tissue thanAnemia in every system. The morphology of airlift-grown gametophytes was similar to that of soil-grown plants; fewer gametophytes with perturbed development were observed in airlift cultures than in the other liquid-based systems. No attempt was made to optimise airlift conditions for the species and tissue employed, so it is concluded that airlift cultivation is a promising system for the bulk production of fern gametophytic tissue. |
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| Keywords: | Ferns Airlift fermenter |
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