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Communities of wood-inhabiting fungi in dead pine logs along a geographical gradient in Japan
Affiliation:1. Laboratory of Forest Ecology, Graduate School of Agricultural Science, Tohoku University, Naruko-onsen, Osaki, Miyagi 989-6711, Japan;2. Center for Ecological Research, Kyoto University, Hirano, Otsu, Shiga 520-2113, Japan;1. Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands;2. Wageningen University, Department of Soil Quality, Droevendaalsesteeg 4, Building 104, 6708 PB Wageningen, The Netherlands;1. College of Pharmacy, Nihon University, Funabashi, Chiba, 274-8555, Japan;2. Department of Environmental Symbiotic Sciences, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan;3. Center for Ecological Research, Kyoto University, Otsu, Shiga, 520-2113, Japan;4. National Institute of Polar Research, Tokyo, 173-8515, Japan;5. Department of Environmental Systems Science, Faculty of Science and Engineering, Doshisha University, Kyoto, 610-0394, Japan;1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan;2. School of Pharmacy, Nihon University, Funabashi, Chiba 274-8555, Japan;3. Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan;4. Department of Environmental Systems Science, Faculty of Science and Engineering, Doshisha University, Kyoto 610-0394, Japan;5. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan;1. School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland Mail Centre, Auckland 1142, New Zealand;2. Landcare Research, Private Bag 92170, Auckland Mail Centre, Auckland 1142, New Zealand;3. Department of Genetics, University of North Carolina, Chapel Hill, NC, USA;4. School of Environment, The University of Auckland, Private Bag 92019, Auckland, New Zealand;5. Fish, Wildlife and Conservation Biology, Colorado State University, Fort Collins, CO, USA;6. Department of Biology, Duke University, Durham, NC 27708, USA
Abstract:Fungi are the main agents of coarse woody debris decomposition in forest ecosystems. We examined the associations of environmental variables with fungal community structures in dead pine logs at 12 geographically distant sites using amplicon pyrosequencing of fungal ITS rDNA. A total of 575 operational taxonomic units (OTUs) were identified based on clustering at 97% similarity. Among the known fungal ecological groups, saprotrophic fungi generally showed highest frequency of occurrence and were positively associated with mean annual temperature (MAT) and log diameter. Wood decay fungi with unknown decay type were positively associated with pine wilt disease and negatively associated with log diameter. Ordination analysis of the 42 most prevalent OTUs showed that MAT and annual precipitation significantly explained the observed fungal community structure. These results suggested that climate conditions and site history differentially effect structure fungal communities in pine logs among different ecological groups.
Keywords:Climate  Dead wood  Decay type  Fungal community  Latitude  Pine wilt disease  Wood decay
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