Paeonol produced by Chaetomium sp., an endophytic fungus isolated from Paeonia suffruticosa |
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| Affiliation: | 1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China;2. Key Laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PRChina;3. Yunnan Institute of Traditional Chinese Medicine and Materia Medica, Kunming, 650223, PR China;1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China;2. School of Chinese Materia medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;1. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, PR China;2. Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, PR China;3. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China;4. Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China;1. Natural Product Chemistry Laboratory, Institute of Chemistry and Chemical Technology, Mongolian Academy of Sciences, 13330 Peace ave., The 4th Building of MAS, Ulaanbaatar, Mongolia;2. Ts. Dash Memorial Laboratory of Bioorganic Chemistry and Pharmacognosy, School of Engineering and Applied Sciences National University of Mongolia, POB-617, Ulaanbaatar 46A, Mongolia;3. Department of Pharmacognosy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan;1. School of Mechanical Engineering, Qingdao Technological University,11 Fushun Road, Qingdao 266033, PR China;2. Department of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China |
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| Abstract: | In order to research the relationship between endophytic fungus and active ingredients in medicinal Paeonia suffruticosa, a total of 57 fungal isolates were isolated from the roots, stems, leaves and buds of medicinal plant Paeonia ostii; mycelium was collected after these fungal isolates were fermented on PDA medium for a few days; then the mycelium products were extracted; their extracts were analyzed by gas chromatography–mass spectrometry. With this method, a strain endophytic fungi named J1-2 which can produce paeonol was screened. Paeonol produced by J1-2 was analyzed by using a high resolution mass spectrometer (HRMS) and nuclear magnetic resonance (NMR). The potential paeonol-procucing named J1-2 was identified Chaetomium based on morphological characteristics and ITS sequence analysis. The current research initially indicates that endophytic fungi can affect the potency of peony. At the same time it also indicates that the numerous endophytic fungi inside the medicinal Paeonia suffruticosa are precious resource for the pharmaceutical natural products that are originally from the Paeonia suffruticosa. |
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| Keywords: | Endophytic fungus Paeonol |
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