Evaluation of the pathogenicity and infectivity of entomopathogenic hypocrealean fungi,isolated from wild mosquitoes in Japan and Burkina Faso,against female adult Anopheles stephensi mosquitoes |
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| Affiliation: | 1. Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa;2. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa;3. Department of Statistics, University of Pretoria, Pretoria 0002, South Africa;4. Department of Plant Sciences, University of the Free State, Bloemfontein, South Africa;1. Instituto Tecnológico El Llano Aguascalientes, Km. 18 Carretera Aguascalientes – San Luis Potosí, El Llano 20330, Aguascalientes, Mexico;2. Facultad de Ciencias Biológicas y Agropecuarias, Universidad de Colima, Tecomán, Colima, Mexico;1. Instituto Federal de Brasília, Brasília, DF, Brazil;2. Embrapa Arroz e Feijão, Rod. GO 462 km 12, Zona Rual, Santo Antônio de Goiás, GO, Brazil;3. Faculdad de Ciencias Medicas, Instituto de Investigaciones Bioquimicas de La Plata (CCT La Plata CONICET-UNLP), La Plata, Argentina;4. Universidade Federal de Goiás, Instituto de Química, Goiânia, GO, Brazil;1. Institute of Zoology, Academy of Sciences of Moldova, Chisinau, Republic of Moldova;2. All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia;3. Institute for Biological Control, Julius Kühn-Institute (JKI), Darmstadt, Germany;4. Institute for Microbiology and Biochemistry, Geisenheim University, Geisenheim, Germany |
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| Abstract: | We investigated the potential of entomopathogenic hypocrealean fungi that naturally occur in or on adult mosquitoes for use as biocontrol agents of vector mosquitoes. The fungi were isolated from wild mosquitoes collected in Japan and Burkina Faso using two isolation methods (with and without surface sterilization). Detected fungal species included Beauveria bassiana sensu lato, Isaria spp., Paecilomyces spp., Lecanicillium spp., and Simplicillium spp. These isolates were used in bioassays against adult female Anopheles stephensi mosquitoes. The median survival time ranged from 5.8 to 14.9 d (control, 17.0 d). Reduced survival times were observed in the isolates from surface-sterilized mosquitoes from Japan, with the isolate B. bassiana s.l. 60-2 exhibiting the highest virulence. This study indicates that adult mosquitoes are naturally infected with various entomopathogenic hypocrealean fungi, and that some of these isolates have the potential for use as fungal pesticides to control vector mosquitoes. |
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| Keywords: | Biological control Fungal isolation Malaria Virulence |
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