弥漫性大B细胞淋巴瘤hs-CRP和VEGF的表达及与化疗耐药的相关性

目的:研究弥漫性大B细胞淋巴瘤超敏C-反应蛋白(hs-CRP)和血管内皮生长因子(VEGF)的表达及与化疗耐药的相关性。方法:选取2013年11月到2014年11月我院收治的化疗耐药弥漫性大B细胞淋巴瘤25例(A组),化疗敏感弥漫性大B细胞淋巴瘤25例(B组),另选取同期健康者25例(C组)。应用免疫荧光法检测三组入选者的hs-CRP,及酶联免疫吸附法试验(ELISA)法检测VEGF。结果:化疗前A组hs-CRP、VEGF水平显著高于B组和C组,B组高于C组,比较差异具有统计学意义(P0.05);化疗缓解后A组和B组hs-CRP、VEGF水平与化疗前比显著降低,差异有统计学意义(P0.05),但两组间比较差异无统计学意义(P0.05);A组复发耐药后hs-CRP、VEGF水平与缓解后比显著升高,差异具有统计学意义(P0.05)。结论:弥漫性大B细胞淋巴瘤血清中hs-CRP、VEGF水平改变与病情变化有关,可能与化疗耐药有关。

Relationship between Expression of hs-CRP and VEGF in Diffuse Large B-cell Lymphoma and Chemotherapy Resistance

Objective:To study the relationship between expression levels of hs-CRP and vas- cular endothelial growth factor (VEGF) in diffuse large B-cell lymphoma and chemotherapy resistance.Methods:A total of 25 cases of diffuse large B-cell lymphoma with chemotherapy resistance were selected as group A; 25 cases of diffuse large B-cell lymphoma with chemotherapy sensitivity were selected as group B; 25 healthy people in the same period, who were all admitted to Huangshi Central Hospital from November 2013 to November 2014 were selected as group C. The hs-CRP levels of three groups were detected by immunofluorescence, and the levels of VEGF, were detected by enzyme-linked immunosorbent assay test (ELISA).Results:The levels of hs-CRP and VEGF in group A were significantly higher than those in group B and group C, those in group B were higher than those in group C before chemotherapy, the differences were statistically significant (P<0.05). Compared to before chemotherapy, the levels of hs-CRP and VEGF in group A and group B significantly decreased after chemotherapy remission, the differences were statistically significant (P<0.05), but the difference between the two groups was not statistically significant (P>0.05). Compared to after chemotherapy remission, the levels of hs-CRP and VEGF in group A significantly increased after recurrence of resistance, the differences were statistically significant (P<0.05).Conclusion:The change of hs-CRP and VEGF levels in diffuse large B-cell lymphoma is related to the patients'' condition changes, which may be associated with chemotherapy resistance.