LRP16影响宫颈癌Siha细胞对化疗药物的敏感性

目的:探讨抑制LRP16的表达对宫颈癌Siha细胞的化疗药物敏感性的影响。方法:将抑制LRP16表达的小干扰RNA:negativecontrol-si RNA(NC)、si RNA-374(si374)转染入Siha宫颈鳞癌细胞系中,通过顺铂(DDP)和紫杉醇(TAX)的处理后,采用CCK-8检测不同浓度紫杉醇、顺铂作用宫颈癌细胞系Siha48 h后,计算出细胞被抑制一半时顺铂、紫杉醇的药物浓度(IC50);使用Hoechst33342染色观察细胞凋亡,采用流式细胞仪检测顺铂IC50作用Siha细胞48小时后的细胞凋亡情况,紫杉醇IC50作用Siha细胞之后的细胞周期分布情况。结果:CCK-8检测转染的Siha细胞增殖活性受到抑制,Hoechst33342染色观察转染的Siha细胞凋亡明显增加,流式细胞仪检测凋亡显示,si374+顺铂的早期凋亡率22.15±2.24,NC+顺铂12.45±2.72,流式细胞仪检测周期显示G2/M(%),si374+紫杉醇29.94±1.87,NC+紫杉醇17.66±2.32。结论:LRP16基因表达下调之后,抑制Siha细胞的增殖、促进其凋亡,使细胞周期滞留于G2/M期,从而提高Siha细胞的化疗敏感性。

Significance of Chemotherapy Sensitivity of the Expression Level ofthe Human LRP16 Gene in Siha Cells

Objective:To investigate the effect of the inhibition of expression of LRP16 in Siha cervical cancer cells onchemotherapeutic drug sensitivity.Methods:Cervical squamous carcinoma cell (Siha cell) was transfected with small interfering RNAwhich was as negative control[siRNA(NC)] and small interfering RNA which was inhibited the expression of LRP16[siRNA-374(si374)],then was treated with cisplatin and paclitaxel. Siha cell was detected by CCK-8 with the treatment of different concentrations of cisplatinand paclitaxel after 48 hours. The drug (cisplatin or paclitaxel) concentration was calculated when Siha cells were half inhibited; Cellmorphology was observated by Hoechst33342 staining. Flow cytometry was utilized to detect the apoptosis of Siha cells after thetreatment of cisplatin IC50 48 hours. G2/M distributionof the cell cycle was detected by flow cytometry after the treatment of paclitaxelIC50 48 hours.Results:The proliferative activity of transfected Siha was inhibited by CCK-8 detection and the apoptosis of transfectedSiha was increased significantly when detectedby Hoechst33342.The early apoptosis rate of siLRP16+cisplatin was 22.15± 2.24, while therate of NC+ cisplatin was 12.45± 2.72 by flow cytometry. The G2/Mdistribution rate of siLRP16+paclitaxel was 29.94± 1.87, while NC+paclitaxel was17.66± 2.32.Conclusion:After the down-regulated expression of LRP16 gene,the proliferation of Siha cells was inhibited,the apoptosis of Siha was induced and the cell cycle remained in the G2/Mphase, thereby enhancing chemosensitivity of Siha cells.