vFLIP激活Jurkat细胞表达HIV抑制因子的研究

目的:检测KSHV编码的vFLIP蛋白对Jurkat细胞HIV抑制因子表达的影响。方法:本研究首先构建真核表达载体pIRES2-EGFP-vFLIP,再将其电穿孔至Jurkat细胞,然后使用实时荧光定量PCR技术检测Jurkat细胞中A3G、A3F、CCL5等HIV抑制因子的表达水平,从而探讨vFLIP抗HIV/AIDS的作用及机制。结果:成功构建了真核表达载体pIRES2-EGFP-vFLIP,电转染效率达到40%左右。与对照载体转染组相比,vFLIP转染组内Jurkat细胞中CCL5、A3G、A3F及Mx2基因的表达分别上调了6.71、2.20、1.52及14.47倍。结论:vFLIP蛋白可以激活Jurkat细胞内某些HIV抑制因子的表达,提示其具有一定的抗HIV/AIDS作用。

vFLIP Stimulates Anti-HIV Factors Expression in Jurkat Cells

Objective:To detect the effect of vFLIP on expression levels of anti-HIV factors in Jurkat cells.Methods:In this study, we constructed the eukaryotic expressive vector of pIRES2-EGFP-vFLIP and transfected the vector to Jurkat cells by electroporation. Then we detected the relative expression levels of anti-HIV factors in vFLIP transfected Jurkat cells by qPCR. Finally, we discussed the functions and mechanisms of vFLIP against HIV/AIDS.Results:The eukaryotic expressive vector of pIRES2-EGFP-vFLIP was successfully constructed. The transfection efficiency of vectors were about 40%. In comparison with pIRES2-EGFP transfected cells, the pIRES2-EGFP-vFLIP transfected cells can increase expression levels of CCL5, A3G, A3F and Mx2 in Jurkat cells by 6.71, 2.20, 1.52 and 14.47 times seperately.Conclusion:vFLIP can activate expressions of CCL5, A3G, A3F and Mx2 in Jurkat cells, and thus suggesting that vFLIP may have the function of anti-HIV/AIDS .