Establishment of <Emphasis Type="Italic">Cyperus aromaticus</Emphasis> cell suspension cultures for the production of Juvenile hormone III |
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Authors: | Lai Keng Chan Pey Shan Lim Mang Ling Choo Peng Lim Boey |
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Institution: | (1) Plant tissue and cell culture laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;(2) School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia |
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Abstract: | Cell suspension cultures of Cyperus aromaticus were established from the yellow friable callus derived from the root explants of in vitro plantlets. Four callus cell lines were selected based on their growth index from two populations of callus cultures originated
from the mother plants grown in two different locations. The selected four cell lines (Z1, Z6, P4, P9) showed uniform cell
growth but produced different amounts of juvenile hormone III (JHIII). The Z1 cell line possessed fast-growing characteristics,
produced a high JHIII content, and was chosen as the elite cell line for an optimization study of C. aromaticus cell suspension cultures. An inoculum cell mass of 0.3 g from 12-d cultures in 30 ml culture medium was found to be the optimum
inoculum size and culture age for establishing the cell suspension culture of C. aromaticus. MS basal medium supplemented with 4.5 mg/l 2,4-D and 5.5 mg/l NAA was found to be the best medium for production of maximum
cell biomass and JHIII. These results indicated that JHIII can be produced from suspension culture of C. aromaticus using a single-stage cell-culture system. |
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