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Preparation of hormone-sensitive adipocytes from foetal rat brown adipose tissue |
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| Authors: | Keith R. F. Elliott Cesar Roncero Margarita Fernandez Manuel Benito |
| Affiliation: | (1) Departamento de Bioquímica, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain;(2) Department of Biochemistry, The Medical School, University of Manchester, M13 9PT Manchester, UK |
| Abstract: | A collagenase-dispersion method involving Percoll gradient separation is described for the production of adipocytes from foetal rat brown adipose tissue. The cells produced are hormone-sensitive as judged by cAMP accumulation and lipolysis. |
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