Effect of various amino acids and ammonium salts in a synthetic culture media on the cholera enterotoxin production |
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Authors: | Ovsova L M Mazrukho A B Lomov Iu M Rozhkov K K Monakhova E K Mikhas' N K Kaminski? D I Kruglikov V D |
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Institution: | Research Institute for Plague Control, Rostov-on-Don, Russia. |
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Abstract: | The influence of amino acids and ammonium salts on the production of cholera enterotoxin (CT) by 3 Vibrio cholerae strains of different biovars and serogroups was evaluated. As revealed in this study, toxin formation in each of the strains was quantitatively and qualitatively determined by their individual sets of amino acids. The amino acid compositions ensuring the maximum production of CT by the V. cholerae strains under study were formed. The use of ammonium salts as the only source of nitrogen in the composition of a synthetic nutrient medium for the accumulation of CT was shown to be inexpedient. |
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