2-Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells |
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| Authors: | H Hibasami M Kawase T Tsukada S Maekawa M Sakurai K Nakashima |
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| Affiliation: | Department of Biochemistry, Mie University School of Medicine, Japan. |
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| Abstract: | Spermidine synthase from rat ventral prostate was inhibited by 2-mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S-adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ. |
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