Plantlet regeneration from mature embryos of Juniperus cedrus |
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| Authors: | Indra S Harry Carolina Martinez Pulido Trevor A Thorpe |
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| Institution: | (1) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alta., Canada;(2) Departmento de Biologia Vegetal, Universidad de la Laguna, Tenerife, Spain |
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| Abstract: | A protocol is described for plantlet regeneration using embryonic explants of Juniperus cedrus Webb & Berth. An average of 6 adventitious buds were induced on whole excised embryos cultured for 15 days on Quoirin and LePoivre (QP) half-strength medium supplemented with 5  M N6-benzyladenine. For bud development, explants were transferred to phytohormone-free 1/2 QP medium. For shoot elongation, explants were cultured on 1/2 QP with 0.05% activated charcoal and 2% sucrose. Adventitious shoots were rooted successfully in peat-vermiculite-perlite (1:1:1) moistened with 1/4 QP containing 1% sucrose and 5 M  -napthaleneacetic acid, pH 5.0. Axillary shoots elongated spontaneously in culture from leaf axils.Abbreviations AC
activated charcoal
- BA
N6-benzyladenine
- NAA
-naphthaleneacetic acid
- QP
Quoirin & LePoivre
- SH
Schenk & Hildebrandt |
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| Keywords: | endangered species Juniper organogenesis |
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