Chromosome stability of cell suspension cultures of Nicotiana spp. |
| |
Authors: | David A. Evans Oluf L. Gamborg |
| |
Affiliation: | (1) DNA Plant Technology Corporation, 2611 Branch Pike, 08077 Cinnaminson, NJ, USA;(2) International Plant Research Institute, 887 Industrial Road, 94070 San Carlos, CA, USA |
| |
Abstract: | Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines derived from Nicotiana tissue with 2n=24, 48, or 72 were established and retain stable chromosome number. Each line could be regenerated to recover plants that retained the somatic chromosome number during culture. Establishment of haploid and diploid cell lines with stable chromosome number is important for mutant isolation and protoplast fusion. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|