Somatic embryogenesis inAesculus |
| |
Authors: | B A Bergmann W P Hackett H Pellett |
| |
Institution: | (1) Department of Forestry, North Carolina State University, Box 8002, 27695-8002 Raleigh, North Carolina;(2) Department of Horticultural Science and Landscape Architecture, University of Minnesota, 55108 Saint Paul, Minnesota |
| |
Abstract: | Summary Somatic embryogenesis was observed with explants taken from four types ofAesculus tissue: (a) shoots of 4-wk-oldin vitro germinated excised embryos (seed fromA.×arnoldiana), (b) roots of 4-wk-oldin vitro germinated excised embryos (seed fromA.×arnoldiana), (c) shoots from newly forced 3-yr-old seedlings (A. glabra), and (d) newly forced shoots from a 30-yr-old tree (A.×arnoldiana “Autumn Splendor”). Shoots provided three types of explants, single node, shoot apex, and internodal section, and all exhibited
embryogenesis.
Proembryogenic masses developed in a few cases after 6 wk in culture but were more commonly seen after 3 mo. The yellow, friable
proembryogenic masses emerged from proximal cut ends of explants. Almost all cultures that formed embryos possessed leaves,
either from developing apical or axillary buds or from adventitious buds, prior to the emergence of proembryogenic masses.
Only tissues that had begun to senesce and had been exposed to cytokinin (benzyladenine at 5 or 25 μM) formed somatic embryos. Embryos with distinct cotyledonlike structures and root/shoot axes developed during the 10 to 16
wk following the inital emergence of proembryogenic masses. Enhanced frequency of embryogenesis was obtained by dark culture
of root and shoot explants from 4-wk-old germinated embryos (A.×arnoldiana) and by dark and cold (5°C) treatment of shoot tissue cultures derived from 3-yr-old seedlings (A. glabra). Embryogenic potential was greatest in the most juvenile tissue and least in the mature tissue. Five percent of shoot explants
taken from the 30-yr-old select treeA.×arnoldiana “Autumn Splendor” produced somatic embryos. |
| |
Keywords: | Aesculus × arnoldiana “ Autumn Splendor” Aesculus glabra buckeye horsechestnut cytokinin |
本文献已被 SpringerLink 等数据库收录! |
|