宁夏密点麻蜥不同部位含药大鼠血清对人胃癌细胞凋亡的影响研究

为探讨宁夏密点麻蜥不同部位含药大鼠血清对人胃癌细胞SGC-7901凋亡影响及其抗癌机制。研究选取SPF级雄性SD大鼠分别以生理盐水、宁夏密点麻蜥不同部位水煎液灌胃,制备含药血清加于胃癌细胞,通过MTT检测细胞活性,AnnexinV-FITC/PI双染法检测细胞凋亡,Western-blot检测胃癌细胞Sirt1和P53蛋白表达。结果显示,宁夏密点麻蜥不同部位各组可明显降低Sirt1和P53蛋白表达水平,抑制细胞增殖,促进凋亡,以尾部组最明显。综上所述,宁夏密点麻蜥不同部位可能通过抑制SIRT1,降低P53,诱导细胞凋亡,其中以宁夏密点麻蜥尾部抗肿瘤作用最显著。

Effects of drugs containing serum from rats feeded with Ningxia dense point lizard different body parts on the proliferarion and apoptosis of human gastric cancer cells

To explore the effects of drugs containing SD rats serum in the different body parts of Ningxia dense point lizard on the apoptosis of the line SGC-7901 human gastric cancer cell,and its anticancer mechanism.The SPF male SD rats were randomly divided into different groups,given corresponding drug intervention to prepare drug containing serum,added to gastric cancer cells,and detected cell viability by MTT,the cell apoptosis with Annexin V-FITC/PI double staining,and the expression of Sirt1 and P53 protein in gastric cancer cells by Western-blot.The results showed that the expression levels of Sirt1 and P53 proteins could be reduced significantly in different parts of the lizard,inhibiting cell proliferation and promoting apoptosis.In summary,different parts of Ningxia dense point lizard may inhibit Sirt1,reduce P53,and induce apoptosis,and the anti-tumor effect of Ningxia dense point lizard tail is the most significant.