| 三叶青对呼吸道合胞病毒作用实验研究 |
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| 引用本文： | 王丹丹,高荣,闫滨.三叶青对呼吸道合胞病毒作用实验研究[J].天然产物研究与开发,2019(6):1070-1074,1100. |
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| 作者姓名： | 王丹丹 高荣 闫滨 |
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| 作者单位： | 山东中医药大学药学院;山东宏济堂制药集团有限公司;山东中医药大学中医学院 |
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| 基金项目： | 科技部“重大新药创制”科技重大专项(2014ZX0950 9001);山东省高等学校中医药抗病毒协同创新中心“中医药抗病毒应用基础与关键技术研究及产业化”首批立项课题(XTCX2014B-01) |
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| 摘 要： | 为探讨三叶青在抗病毒中的作用,本文选取三叶青乙醇提取物正丁醇萃取部分和乙酸乙酯萃取部分,采用细胞病变抑制实验,以治疗指数(TI)为研究指标,研究各溶剂萃取部位、柱分离部分在MA104细胞中对呼吸道合胞病毒(respiratory syncytial virus,RSV)的抑制作用,从而筛选出三叶青抗RSV有效部位,分离活性成分。三叶青乙醇提取物正丁醇萃取部分和乙酸乙酯萃取部分的TI值分别为128和64,相对于阳性对照药利巴韦林(TI值为6.25)。说明三叶青乙醇提取物正丁醇萃取部分和乙酸乙酯萃取部分具有抗RSV的活性且明显优于利巴韦林。
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| 关 键 词： | 三叶青 呼吸道合胞病毒 治疗指数 体外抗病毒 |
Experimental study on the effects of Radix Tetrastigmae on respiratory syncytial virus |
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| WANG Dan-dan,GAO Rong,YAN Bin.Experimental study on the effects of Radix Tetrastigmae on respiratory syncytial virus[J].Natural Product Research and Development,2019(6):1070-1074,1100. |
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| Authors: | WANG Dan-dan GAO Rong YAN Bin |
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| Institution: | (College of Pharmacy,Shandong University of Traditional ChineseMedicine;Shandong Hongjitang Pharmaceutical Group;College of Traditional Chinese Medicine,Shandong University ofTraditional Chinese Medicine,Jinan 250355 ,China) |
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| Abstract: | In order to explore the antiviral effect of Radix Tetrastigmatis hemsleyani,we continue to choose n-Rutanol and Ethyl acetate extractions from Radix Tetrastigmatis hemsleyani’s ethanol extract to investigate their anti RSV activity by method of cytopathic effect(CPE) and the therapeutic index(TI).MA104 cell was used in this experiment.Thereby we can find out the effective fraction of Radix Tetrastigmatis hemsleyani against RSV and isolate the active ingredients.Results showed that the TI of n-Rutanol and Ethyl acetate extraction are 128 and 64 respectively,and the TI of ribavirin is 6.25,which has a significant inhibitory effect on RSV.The results indicated that n-Rutanol and Ethyl acetate extraction of Radix Tetrastigmatis hemsleyani had antiviral activity against RSV and were obviously superior to ribavirin. |
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| Keywords: | Radix Tetrastigmatis hemsleyani respiratory syncytial virus Therapeutic index Antivirus in vitro |
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