基于绿色低共熔溶剂法高效提取鸡骨草中的黄酮和皂苷

为了研究鸡骨草中总黄酮和总皂苷的最佳提取工艺,通过单因素试验和星点设计-响应面法,对低共熔溶剂的性质(如种类、组分摩尔比、含水量)、提取温度、提取时间、液料比等多个因素进行考察。结果显示,总黄酮的最佳提取条件为:摩尔比1∶2、含水量30%的氯化胆碱/乙二醇作溶剂,提取温度80℃,提取时间40min,液料比15∶1(mL/g);总皂苷的最佳提取条件为∶摩尔比1∶4、含水量25%的氯化胆碱/乳酸作溶剂,提取温度80℃,提取时间64min,液料比56∶1(mL/g)。在最佳条件下,总黄酮和总皂苷的提取率较传统提取溶剂分别提高了33.3%和96.4%。本研究为鸡骨草中黄酮和皂苷的高效、安全提取提供了一条新思路。

Green and efficient extraction of flavoniods and saponins from Abrus cantoniensis Hance by deep eutectic solvents

In order to research the optimal extraction process of total flavoniods and total saponins from Abrus cantoniensis Hance.Properties of DESs (including type,component mole ratio and water content),extraction temperature,extraction time,and liquid-solid ratio were studied by using single factor experiment and Box-Behnken experimental design combined with responsesurface methodology(BBD-RSM).The results indicated that the optimal extraction conditions for total flavoniods were as follows:ChCl/ethylene glycol with mole ratio of 1 ∶2 and water content of 30% as solvent,extraction temperature of 80 ℃,extraction time of 40 min,and liquid-solid ratio of 15 ∶1 (mL/g).The optimum conditions for total saponins were as follows:ChCl/lactic acid with mole ratio of 1 ∶4 and water content of 25% as solvent,extraction temperature of 80 ℃,extraction time of 64 min,liquid-solid ratio of 56 ∶1 (mL/g).Under the optimum conditions,the extraction yields of total flavoniods and total saponins increased by 33.3% and 96.4%,respectively,compared with conventional extraction solvents.The research could provide a new idea for efficient and safe extraction of flavoniods and saponins from Abrus cantoniensis Hance.