| 八种石斛中苯丙醇类和香豆素类化合物含量测定与分析 |
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| 引用本文： | 师亦洁,周峰旭,李鲜,范宇超,刘宏程. 八种石斛中苯丙醇类和香豆素类化合物含量测定与分析[J]. 天然产物研究与开发, 2019, 0(5): 803-807,819 |
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| 作者姓名： | 师亦洁 周峰旭 李鲜 范宇超 刘宏程 |
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| 作者单位： | 昆明医科大学药学院暨云南省天然药物药理重点实验室;农业部农产品质量监督检验测试中心(昆明)云南省农业科学院质量标准与检测技术研究所 |
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| 基金项目： | 国家重点研发计划(2016YFF0201806);云南省科技惠民计划(2014DA001) |
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| 摘 要： | 新建HPLC双波长同时测定松柏苷、紫丁香苷、东莨菪内酯、滨蒿内酯及香豆素含量的分析方法。采用Shiseido CAPCELL PAK MGⅡC18柱(250 mm×4.6 mm,5μm)分析柱;使用乙腈-水为流动相,梯度洗脱;流速为0.8 mL/min;紫外检测器260 nm,315 nm双波长检测。结果显示,五种成分在测定范围内有良好的线(r^2>0.999);在三个加标浓度下方法回收率为98.7%～102.5%之间,相对标准偏差(RSD)小于2.5%。该方法用于8种石斛品种含量的测定。
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| 关 键 词： | 石斛 苯丙醇类 香豆素类 HPLC |
Determination and analysis of phenylpropanol and coumarin compounds in eight kinds of Dendrobium |
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| SHI Yi-jie,ZHOU Feng-xu,LI Xian,FAN Yu-chao,LIU Hong-cheng. Determination and analysis of phenylpropanol and coumarin compounds in eight kinds of Dendrobium[J]. Natural Product Research and Development, 2019, 0(5): 803-807,819 |
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| Authors: | SHI Yi-jie ZHOU Feng-xu LI Xian FAN Yu-chao LIU Hong-cheng |
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| Affiliation: | (School of Pharmaceutical Science & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University,Kunming 650500,China;Supervision and Testing Center for Farm Product Quality,Ministry of Agriculture,Institute of Quality Standard and Testing Technology,Yunnan Academy of Agricultural Science,Kunming 650223,China) |
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| Abstract: | A new method for simultaneous determination of cedarin,syringin,scutellarin,scoparone and coumarin was devel- oped. The method was analyzed by Shiseido CAPCELL PAK MG II C18 column ( 250 mm × 4. 6 mm,5 μm),and with a gradient solvent system of water-acetonitrile. The flow was set at 0. 8 mL/min. The UV detection was set with double wave- lengths of 260 nm and 315 nm. The method was validated and found to be satisfactorily linear ( r2 > 0. 999). Recoveries ranged from 98. 7% to 102. 5% for all compounds at three different spiking levels and relative standard deviation ( RSD) was with 2. 5% or less. The established method was successfully used for evaluating the quality in eight kinds of Dendrobium. |
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| Keywords: | Dendrobium phenylpropanols coumarins HPLC |
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