山楂叶金丝桃苷对高糖诱导SH-SY5Y细胞损伤的保护作用及机制

为研究金丝桃苷对高糖诱导的人神经母细胞瘤(SH-SY5Y)细胞氧化损伤的保护作用及机制,用含100mmo L/L葡萄糖和分别为20、50、100μmo L/L金丝桃苷的培养基共同孵育SH-SY5Y细胞36 h,检测细胞活力、细胞培养液中乳酸脱氢酶(LDH)水平及半胱氨酸天冬氨酸蛋白酶-3(caspase-3)活性,细胞内活性氧(ROS)水平、丙二醛(MDA)、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及SIRT1和NF-кB基因的mRNA水平和蛋白含量。结果显示金丝桃苷可提高高糖诱导后SH-SY5Y细胞的存活率,抑制细胞LDH释放,清除ROS,降低MDA含量与caspase-3活性,增强SOD、CAT活性和GSH含量;同时,金丝桃苷还能提高SIRT1基因的mR-NA表达及蛋白含量,降低NF-кB基因的mRNA水平和蛋白含量。结果表明金丝桃苷能通过激活SIRT1基因,抑制NF-кB基因保护高糖所致SH-SY5Y细胞的氧化损伤。

Protective effects of Chinese hawthorn leaf hyperoside against high glucose-induced injury in SH-SY5Y cells

This study was to explore the protective effect and mechanisms of hyperoside(Hyp)against oxidative stress of SH-SY5Y cells induced by high glucose.SH-SY5Y cells in 100 mmol/L glucose medium was incubated with 20,50,100 μmoL/L hyperoside for 36 h.The cell proliferation of SH-SY5Y,the levels of lactate dehydrogenase(LDH),intracellular reactive oxygen species(ROS)as well as the contents of malondialdehyde(MDA)and glutathione(GSH),activities of caspase-3,superoxide dismutase(SOD)and catalase(CAT)were measured.Both mRNA expression levels and protein expression levels of NF-кB and SIRT1 genes were detected.The treatment of SH-SY5Y cells with hyperoside had a marked diminution in LDH level,caspase-3 activity,intracellular levels of MDA and ROS.Whereas,the cell viability,the activities of SOD and CAT,GSH contents,the mRNA expressions and the protein contents of SIRT1 were notably elevated and the mRNA and the protein expression of NF-кB were attenuated by hyperoside.The results indicated that hyperoside may protect against the oxidative damage of SH-SY5Y cells induced by high glucose via the activation of SIRT1 and inhibition NF-кB.