枸杞色素及多糖的不同提取次序对其得率及抗氧化活性的影响

为探究先后提取枸杞多糖及枸杞色素时对各自得率的影响。本研究通过分别考察这两种成分在提取过程中的提取溶剂、提取温度、料液比、提取次数及提取时间等因素对枸杞色素和枸杞多糖的得率影响,确定枸杞色素和枸杞多糖在提取次序不同时,两者的最佳提取工艺以及对DPPH·和·OH自由基清除率。结果表明,首先提取枸杞多糖后,枸杞色素的最佳提取工艺为采用正己烷,80℃时,料液比1∶10,提取2次,每次1 h,枸杞色素得率为2.48%,此时枸杞多糖得率为7.45%;而首先提取枸杞色素后,采用了超声辅助提取的方式提取枸杞多糖,发现超声效率为25%,料液比1∶10,提取20 min,枸杞多糖得率为5.23%,此时枸杞色素得率为3.93%。因此,首先提取枸杞多糖,使其平均得率为7.45%,而后提取枸杞色素,其平均得率为2.48%;总体上,枸杞色素1和枸杞多糖1对DPPH·自由基清除率都较高,枸杞多糖1对·OH自由基清除率较高,其抗氧化活性都接近Vc。

Effect of different extraction order on the yield of Lycium Barbarum pigment and polysaccharides and their antioxidant activities

In order to explore the influence of different extraction order of Lycium barbarum polysaccharides and Lycium barbarum pigment on their yield,this paper will research respectively the effects of solvent,temperature,ratio of material to liquid,times of extraction and extraction time on the yield of Lycium barbarum polysaccharides and Lycium barbarum pigment,and the optimum extraction conditions of different order and their free radical scavenging rate of DPPH·and ·OH were determined.The results showed that the best extraction technology of Lycium barbarum pigment was n-hexane as the solvent,80 ℃,the ratio of material to liquid was 1∶10,extraction time was 1 h,and the yield of pigment was 2.48% and the yield of polysaccharides was 7.45% when extracted polysaccharides as first;the optimum extraction technology of Lycium barbarum polysaccharides was ultrasonic efficiency as 25%,the ratio of material to liquid was 1∶10,and extracted for 20 min with ultrasonic assisted extraction,and the yield of polysaccharides was 5.23% and the yield of polysaccharides was 3.93% when extracted pigment as first.Consequently,the average yield of Lycium barbarum polysaccharide was 7.45%,and pigment was 2.48% when firstly extracted polysaccharide.In general,Lycium barbarum pigment 1 and Lycium barbarum polysaccharide 1 have higher free radical scavenging rate of DPPH·and Lycium barbarum polysaccharide 1 has higher free radical scavenging rate of ·OH,and their antioxidant activities were close to Vc.