怀地黄叶片中总环烯醚萜苷及总苯乙醇苷含量快速分析模型的建立

本文旨在建立地黄叶片中总环烯醚萜苷及苯乙醇苷定量分析模型。利用紫外-可见分光光度法测定不同种质怀地黄生育期内的128份地黄叶片中总环烯醚萜苷及总苯乙醇苷的含量,并将其作为基础值,结合地黄叶片的近红外光谱图,利用TQ8.0分析软件结合偏最小二乘法(PLS),分别建立地黄叶片中总环烯醚萜苷及总苯乙醇苷的定量分析模型。地黄叶片中总苯乙醇苷定量校正模型决定系数(R2)为0.998 2,校正均方根偏差(RMSEC)为0.089 9,预测均方决定差(RMSEP)为0.142,交叉验证均方根偏差(RMSECV)为0.707 2;总环烯醚萜苷定量校正模型的内部交叉验证决定系数(R2)为0.972 1,校正均方差(RMSEC)为0.259,预测均方决定差(RMSEP)为0.095 4,交叉验证均方根偏差(RMSECV)为0.869 4。预测值与实测值差异无统计学意义。该定量模型可用于怀地黄叶片中总环烯醚萜苷及总苯乙醇苷含量的快速测定。

Establishment of rapid analytical models for total iridoid glycosides and total phenethyl alcohol glycosides in leaves of Rehmannia glutinosa

This paper aimed to establish a quantitative analysis model for total iridoid glycosides and phenylethanoid glyco- sides in leaves of Rehmannia glutinosa. The content of total iridoid glycosides and phenylethanoside in 128 samples of Reh- mannia glutinosa leaves with different texture and different growth stages were determined by UV-Vis spectrophotometry,and it was used as the base value,combined with the near infrared spectra of leaves of Rehmannia glutinosa. The quantitative anal- ysis models of total iridoid glycosides and phenylethanoside in leaves of Rehmannia glutinosa were established by using TQ 8. 0 analysis software and partial least squares ( PLS). The internal cross validation determination coefficient ( R2 ) was 0. 998 19,the correction mean variance ( RMSEC) was 0. 089 9,the root mean determination coefficient ( R2 ) and the correction mean variance ( RMSEC) of the quantitative calibration model for total iridoid glycosides in leaves of Rehmannia glutinosa were 0. 972 1 and 0. 125 9 respectively. The root mean square error predication was 0. 095 4 and the root mean square error of cross validation( RMSECV) was 0. 869 4. There was no significant difference between the predicted value and the actual val- ue. The quantitative model can be used to determine the content of total iridoid glycosides and total phenylethanol glycosides in leaves of Rehmannia glutinosa.