不同方法提取杜仲中桃叶珊瑚苷等4种高活性成分的比较研究

为研究生物化学方法对杜仲中高活性成分的提取率,本文以高效液相色谱法检验样品中提取出活性成分的含量差异,比较了在相同条件下酶解法、水提法、醇提法对杜仲树皮中桃叶珊瑚苷、京尼平苷、京尼平苷酸与绿原酸这4种活性成分的提取量,并对4种活性成分的不同提取效果进行了比较研究。结果发现三种方法的提取量存在明显差异,其中桃叶珊瑚苷提取量为:酶解法22. 42μg/g,水提法8. 27μg/g,醇提法9. 13μg/g;京尼平苷提取量为:酶解法77. 89μg/g,水提法33. 19μg/g,醇提法7. 76μg/g;京尼平苷酸提取量为:酶解法110. 05μg/g,水提法36. 63μg/g,醇提法47. 40μg/g;绿原酸提取量为:酶解法345. 35μg/g,水提法118. 85μg/g,醇提法172. 04μg/g,即酶解法提取量均比水提法、醇提法高出数倍。实验表明酶解法是3种方法中提取效果最好的,且无化学污染。

Comparative study on extraction of 4 highly active ingredients such as aucalein from Eucommia ulmoides Oliv.

In order to study the extraction rate of high-activity components in Eucommia ulmoides by biochemical methods,this paper compared the enzymatic hydrolysis,water extraction and alcohol extraction methods in the eucommia bark of the eucommia bark,genipin,genipin The extraction of four active components,acacia,genipin,genipin and chlorogenic acid,was carried out by high performance liquid chromatography to determine the difference in the content of active ingredients extracted from the samples.The extraction effects of four active ingredients in the extraction of Eucommia bark from different extraction methods were compared and compared.The determination of the extraction quantity of the 4 natural compounds with three solutions respectively by liquid chromatography,which showed that there were significant differences in contents between three extracting solutions.The contents of Aucubin were 22.42 μg/g with aqueous solution containing enzyme,8.27 μg/g with distilled water,and 9.13 μg/g with 70% ethanol solution.The contents of Geniposide were 77.89 μg/g with aqueous solution containing enzyme,33.19 μg/g with distilled water and 7.76 μg/g with 70% ethanol solution.The contents of Geniposidic acid were 110.05 μg/g with aqueous solution containing enzyme,36.63 μg/g with distilled water and 47.40 μg/g with 70% ethanol solution.The contents of Chlorogenic acid were 345.35 μg/g with aqueous solution containing enzyme,118.85 μg/g with distilled water and 172.04 μg /g with 70% ethanol solution.That is,the amount of extraction by enzymatic hydrolysis is several times higher as much as that of water extraction and ethanol extraction.Those determination results showed that the enzymatic hydrolysis was the best among the three methods in natural products extraction,and also enzymatic extraction causes no any chemical pollution.